Protein Kinase C, zeta, Mid (PKCz) (Biotin)

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Recognizes mouse PKCz (~70kD) and PKMz (~55kD) proteins. The brain-specific isoform of this protein (PKMz) may appear as a doublet at ~55kD in Western Blot of brain homogenates. In mouse kidney homogenate, it identifies the band at ~70kD. Reactivity has been confirmed with mouse kidney and brain homogenates.