Prostate-derived Ets Factor (PDEF) (HRP)

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Specific for human PDEF. On Western blots, it identifies a single band at ~37.5kD using LNCAP lysates. There is also a weak band at ~55kD which may be due to the glycosylated form of PDEF. Reactivity has been confirmed with human LNCAP prostate carcinoma cell lysates.