Prostate-derived Ets Factor (PDEF) (Biotin)

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Specific for human PDEF. On Western blots, it identifies a single band at ~37.5kD using LNCAP lysates. There is also a weak band at ~55kD which may be due to the glycosylated form of PDEF. Reactivity has been confirmed with human LNCAP prostate carcinoma cell lysates.