PAK1 (p21 GTPase activated protein kinase 1) (HRP)

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Specific for the 68kD PAK1 (alphaPAK) protein and does not react with the closely related PAK2 (gammaPAK) or PAK3 (betaPAK) proteins. Reactivity of this antibody has been confirmed by Western blotting using lysates derived from NIH 3T3 cells, Jurkat cells, and COS-7 cells transfected with a PAK1 expression vector. Reacts with PAK1 from human, mouse and rat.