Anti-BSEP (Bile Salt Export Pump) Antibody

Mouse Monoclonal Antibody specific to BSEP

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

ATP-binding cassette sub-family B member 11

Gene Name

ABCB11

NCBI Gene ID

UniProt

O95342

Cellular Locus

Apical cell membrane, Recycling endosome membrane, Endosome, Cell membrane

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human BSEP.

Target Antigen

Bile salt export pump

Target

BSEP (Bile Salt Export Pump)

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

IHC

Field of Research

Cancer research

Purification Method

Purified by immunoaffinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Tris

Function

Catalyzes the transport of the major hydrophobic bile salts, such as taurine and glycine-conjugated cholic acid across the canalicular membrane of hepatocytes in an ATP-dependent manner, therefore participates in hepatic bile acid homeostasis and consequently to lipid homeostasis through regulation of biliary lipid secretion in a bile salts dependent manner (PubMed:16332456, PubMed:22262466, PubMed:15791618, PubMed:18985798, PubMed:19228692, PubMed:20398791, PubMed:24711118, PubMed:29507376, PubMed:20010382, PubMed:32203132). Transports taurine-conjugated bile salts more rapidly than glycine-conjugated bile salts (PubMed:16332456). Also transports non-bile acid compounds, such as pravastatin and fexofenadine in an ATP-dependent manner and may be involved in their biliary excretion (PubMed:15901796, PubMed:18245269). {PubMed:15791618, PubMed:15901796, PubMed:16332456, PubMed:18245269, PubMed:18985798, PubMed:19228692, PubMed:20010382, PubMed:20398791, PubMed:22262466, PubMed:24711118, PubMed:29507376, PubMed:32203132}.

Additionnal Information

<br><br>Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

Human BSEP. Reactivity with other species has not been investigated.

Formulation

Tris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide.

Buffer pH

pH 7.3-7.7

Target Background

Bile Salt Export Pump (BSEP) is a member of the ATP-binding cassette transporters which mediate the transport of bile acid, taurocholate, and other cholate conjugates across the hepatocyte canalicular membrane into the canaliculus. BSEP is associated with progressive familial intrahepatic cholestasis type 2 (PFIC2) and benign recurrent intrahepatic cholestasis type 2 (BRIC2). PFIC2 caused by mutations in the BSEP gene appears to increase the risk of hepatocellular carcinoma in early life.