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Recombinant Human GSTAL Protein (Trx Tag)

Product Specifications

Background

GSTA1 (Glutathione S-Transferase Alpha 1) is a Protein Coding gene. This gene encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds. Glutathione S-transferases (GSTs) are involved in the detoxification of carcinogens and may be linked to carcinogenesis. As a vital component of GSTs, GSTA1 plays an important role in carcinogenesis. GSTA1 expression may be a target molecule in the early diagnosis and treatment of lung cancer. Human colonic adenocarcinoma (Caco-2) cells in culture undergo spontaneous differentiation into mature enterocytes in association with progressive increases in expression of glutathione S-transferase alpha-1 (GSTA1) . GSTA1 levels may play a role in modulating enterocyte proliferation but do not influence differentiation or apoptosis. GSTA1 may play a key role during pregnancy.

Abbreviation

GSTAL

Synonyms

GST; GTH; GSTA; HA subunit; GST HA subunit; GST class alpha member; GST class-alpha member; Glutathione S transferase A; Glutathione S alkyltransferase A; S (hydroxyalkyl) glutathione lyase A; GSTA1; GST-epsilon; GST2; GSTA1-1; GTH1; Glutathione S alkyltransferase A1; GST class-alpha member 1; HA subunit 1; S (hydroxyalkyl) glutathione lyase A1; Glutathione S transferase A1; GST class alpha member 1; GST epsilon; GST HA subunit 1; GSTA1 1; Glutathione S transferase 2; Glutathione S transferase Ha subunit 1; GST class-alpha member 1; GST epsilon; GST HA subunit 1; GST2; GSTA1; GSTA1-1; GST-epsilon; GTH1; HA subunit 1; MGC131939

UniProt

P08263

Accession Number

P08263

Expression System

E.coli

Tag

N-Trx

Sequence

Met1-Phe222

Field of Research

Signal Transduction; Tags & Cell Markers; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

44.4 kDa

Observed Molecular Weight

39 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human

Available Sizes

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