Hakai (APC)

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recognizes canine Hakai. On Western blots, it identifies a single band at ~70kD, as well as a band at ~75-80kD that likely represents the ubiquitinated form of the Hakai protein. Reactivity has been confirmed with MDCK cell lysates. Species sequence homology: Mouse and human.