Endophilin, pan, SH3 Domain (Biotin)

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Reacts with mouse endophilin 1, 2 and 3 proteins. Based on 100% sequence homology, it is expected to cross-react with human and rat samples. On Western blots of mouse brain homogenates, two bands at ~40kD (endophilin 1, 2) and one band at ~45kD (endophilin 3) are identified. A faint band at ~52-55kD is also observed, which may represent an isomer. Reactivity has been confirmed with mouse brain lysates.