SARS-CoV-2 Spike RBD Protein (S477N, E484K), His Tag (MALS verified)

SARS-CoV-2 Spike RBD (S477N, E484K), His Tag (SPD-C52Hq) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (S477N, E484K)). The mutations (S477N, E484K) were identified in the SARS-CoV-2 variant B.1.620, which was found to circulate in Europe and Central Africa.

Product Specifications

Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.5 kDa. The protein migrates as 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Arg 319 - Lys 537

Host

HEK 293

Target

Spike RBD

Conjugation

Unconjugated

Tag

C-10×His

Source

SARS-CoV-2

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized SARS-CoV-2 Spike RBD (S477N, E484K), His Tag (Cat. No. SPD-C52Hq) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-2 ng/mL (QC tested).