a-Catenin (AP)

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Specific for a-catenin, a 102kD protein. Does not crossreact with the related b-catenin or g-catenin proteins. Species Crossreactivity: Human, Mouse, Rat, Chicken, Xenopus. Tissues/Lysates Tested: HeLa cells, A431 cells, WI-38 human fibroblasts, Xenopus Cytosol, NIH 3T3 cells.