FITC-Labeled Human Glypican 3 / GPC3 Protein, His Tag
FITC-Labeled Human Glypican 3, His Tag (Cat. No. GP3-HF2H1) is expressed from human HEK293 cells. It contains AA Gln 25 - His 559 (Accession # P51654-1) . It is the FITC labeled form of Human Glypican 3, His Tag (Cat. No. GP3-H52H4) .
Product Specifications
Background
Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 38.1 kDa & 24.6 kDa. The protein migrates as 30 kDa, 38-40 kDa, and 65-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P51654-1
Expression Region
Gln 25 - His 559
Host
HEK293
Target
Glypican 3
Conjugation
FITC Labeled
Tag
C-10xHis
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized Anti-hGPC3 antibody (Human IgG1) at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human Glypican 3, His Tag (Cat. No. GP3-HF2H1) with a linear range of 0.078-0.625 μg/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
38.1 kDa & 24.6 kDa
Shipping Conditions
RT
Storage Conditions
-20°C, aviod light
Package Size
200ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/GP3-HF2H1.pdf
Species
Human
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