TregFlowEx Kit

ED7417-1 Fix and Lysing Solution – 1 vial containing 10 ml of 10x concentrated reagent. ED7417-2 Permeabilizing Solution – 1 vial containing 25 ml of reagent, ready-to-use. ED7417-3 Blocking Buffer – 1 vial containing 2.5 ml of reagent, ready-to-use. ED7417-4 CD4 FITC/CD25 PE – 1 vial containing 0.50 ml of cell surface staining reagent, a mixture of mouse monoclonal antibody against CD4, FITC labelled (clone MEM-241, isotype IgG1) and antibody against CD25, RPE labelled (clone MEM-181, isotype IgG1) . ED7417-5 FOXP3 APC – 1 vial containing 0.25 ml of monoclonal antibody against FOXP3, APC labelled (clone 3G3, isotype IgG1) .

*Hrdý J, Vlasáková K, Černý V, Súkeníková L, Novotná O, Petrásková P, Boráková K, Lodinová-Žádníková R, Kolářová L, Prokešová L: Decreased allergy incidence in children supplemented with E. coli O83:K24:H31 and its possible modes of action. Eur J Immunol. 2018 Dec;48 (12) :2015-2030. , URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=hrdy+j+vlasakova+k, *Viktor Cerny, Jiri Hrdy, Olga Novotna, Petra Petraskova, Kristyna Borakova, Libuse Kolarova, Ludmila Prokesova: Distinct characteristics of Tregs of newborns of healthy and allergic mothers. PLoS One. 2018 Nov 26;13 (11) :e0207998. , URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0207998, *Černý V, Novotná O, Petrásková P Hudcová K, Boráková K, Prokešová L, Kolářová L, Hrdý J: Lower functional and proportional characteristics of cord blood treg of male newborns compared with female newborns. Biomedicines 2021, 9, 170., URL: https://pubmed.ncbi.nlm.nih.gov/33572097/