Human NFL Protein, His Tag
Human NFL, His Tag (NFL-H5143) is expressed from E. coli cells. It contains AA Ser 2 - Asp 543 (Accession # P07196-1).
Product Specifications
Background
Neurofilaments (NF) are classed as type IV intermediate filaments found in the cytoplasm of neurons. Together with microtubules and microfilaments, they form the neuronal cytoskeleton. Neurofilaments usually contain three intermediate filament proteins: NFL, NFM, and NFH which are involved in the maintenance of neuronal caliber. Neurofilament heavy (NFH) can server as biomarkers for several diseases by indicating axonal injury.
Specifications
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 63.4 kDa.
Expression Region
Ser 2 - Asp 543
Host
E. coli
Target
NFL
Conjugation
Unconjugated
Tag
N-10×His
Source
Human
Stability
The product MUST be stored at -70°C or lower upon receipt; -70°C for 12 months under sterile conditions.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized Human NFL, His Tag (Cat. No. NFL-H5143) at 1 μg/mL (100 μL/well) can bind Anti NEFL Antibody with a linear range of 1-16 ng/mL (QC tested).
Format
Liquid
Buffer
PBS, pH7.4
Additives
Glycerol
Molecular Weight
63.4 kDa
Shipping Conditions
Dry ice
Storage Conditions
-70°C
Documents
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