Recombinant Fasciola hepatica Cathepsin L2, partial

Product Specifications

Abbreviation

Recombinant Fasciola hepatica Cathepsin L2 protein, partial

UniProt

P80342

Expression Region

1-14aa

Organism

Fasciola hepatica (Liver fluke)

Target Sequence

AVPDKIDRRESGYV

Tag

N-terminal GST-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Signal Transduction

Relevance

Thiol protease that assists the parasite in burrowing through the gut wall and liver of its mammalian host.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

28.3 kDa

References & Citations

"Purification and characterisation of a second cathepsin L proteinase secreted by the parasitic trematode Fasciola hepatica." Dowd A.J., Smith A.M., McGonicle S., Dalton J.P. Eur. J. Biochem. 223:91-98 (1994)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.