Rat Nectin-4 Protein, Fc Tag (MALS verified)
Rat Nectin-4, Fc Tag (NE4-R5255) is expressed from human 293 cells (HEK293) . It contains AA Tyr 28 - Val 348 (Accession # D3ZET1-1) .
Product Specifications
Background
Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. Nectins are cell adhesion molecules that play a key role in various biological processes such as polarity, proliferation, differentiation and migration, for epithelial, endothelial, immune and neuronal cells, during development and adult life. Nectin-4 is a tumor-associated antigen in 50%, 49% and 86%o of breast, ovarian and lung carcinomas, respectively, mostly on tumors of bad prognosis. Its expression is not detected in the corresponding normal tissues.
Specifications
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 61.1 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
D3ZET1-1
Expression Region
Tyr 28 - Val 348
Host
HEK293
Target
Nectin-4
Conjugation
Unconjugated
Tag
C-Fc
Source
Rat
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
0.1 EU per μg
Purity
95%
Bioactivity
Immobilized Rat Nectin-4, Fc Tag (Cat. No. NE4-R5255) at 1 μg/mL (100 μL/well) can bind Anti-Nectin-4 Antibody, Mouse IgG1 with a linear range of 0.039-0.625 μg/mL (QC tested).
Format
Powder
Buffer
50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
Additives
Trehalose
Molecular Weight
61.1 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
100ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/NE4-R5255.pdf
Species
Rat
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