Phosphothreonine (AP)

Recognizes Phosphothreonine. Reacts specifically with proteins containing phosphorylated threonine residues (phosphothreonine). Recognition of phosphothreonine containing proteins by this antibody appears to be independent of neighboring amino acids and species of origin of the phosphorylated protein. No significant crossreactivity with phosphoserine, phosphotyrosine either as free amino acids or in proteins has been observed. Has been confirmed to react with phosphothreonine containing proteins from lysates derived from EGF-stimulated A431 cells, HeLa cells, MCF-7 cells and Clone 9 (rat liver) cells.