Anti-α-1-antitrypsin Mouse Monoclonal Antibody - Library Pack

Selection of Mouse Monoclonal antibodies specific to α-1-antitrypsin

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-α-1-antitrypsin Mouse Monoclonal Antibody Library Pack - Includes 100 ug of each of the following antibodies: 13701 - Anti-α-1-antitrypsin Mouse Monoclonal Antibody - Clone 1AT10-105.10 - Isotype IgG2a 13702 - Anti-α-1-antitrypsin Mouse Monoclonal Antibody - Clone 1AT11-210.1 - Isotype IgG1 13703 - Anti-α-1-antitrypsin Mouse Monoclonal Antibody - Clone 1AT11-209.3 - Isotype IgG1 13704 - Anti-α-1-antitrypsin Mouse Monoclonal Antibody - Clone 1AT11-213.5 - Isotype IgG1

Gene Name

SERPINA1

Gene ID

107400

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=SERPINA1">SERPINA1</a>

UniProt

P01009

Cellular Locus

Secreted. Endoplasmic reticulum. Note=The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum.; [Short peptide from AAT]: Secreted, extracellular space, extracellular matrix.

Host

Mouse

Reactivity

Human

Immunogen

Purified human serum alpha-1-antitrypsin.

Target Antigen

α-1-antitrypsin

Target

α-1-antitrypsin

Clonality

Monoclonal

Type

Antibody Library Pack

Applications

ELISA

Field of Research

Enzymes

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.; [Short peptide from AAT]: Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

Additionnal Information

These antibodies have been qualified for use in ELISA to detect human a1AT. End users should determine optimal concentrations for their applications.

Storage Conditions

These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Specificity

These antibodies specifically recognize human serum alpha-1-antitrypsin. They do not cross-react with alpha-1-antichymotrypsin.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Alpha-1 Antitrypsin (alpha1AT) is a protease inhibitor of the serpin superfamily. It is generally known as serum trypsin inhibitor and is also referred to as alpha-1 proteinase inhibitor (alpha1PI) because it inhibits multiple proteases. It protects tissues from proteases of inflammatory cells, particularly neutrophil elastase, and has a reference range in blood of 1.5 â€“ 3.5 gram/liter, but the concentration can rise significantly in cases of acute inflammation. In its absence (such as in alpha1AT deficiency), neutrophil elastase is free to break down elastin, which contributes to the elasticity of the lungs, resulting in respiratory complications such as emphysema, or COPD (chronic obstructive pulmonary disease) in adults and cirrhosis in adults or children.