RNA and DNA Endonuclease from Serratia marcescens

<strong>RNA and DNA Endonuclease from Serratia marcescens</strong>_x000D_ <strong>Catalog number:</strong> B2014452_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 1 kU_x000D_ <strong>Molecular Weight or Concentration:</strong> N/A_x000D_ <strong>Supplied as:</strong> Solution_x000D_ <strong>Applications:</strong> molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> −20°C_x000D_ <strong>Keywords:</strong> Endonucleaase_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Menacho-Melgar R, Moreb EA, Efromson JP, Yang T, Hennigan JN, Wang R, Lynch MD. Improved two-stage protein expression and purification via autoinduction of both autolysis and auto DNA/RNA hydrolysis conferred by phage lysozyme and DNA/RNA endonuclease Biotechnol Bioeng. 2020 Sep;117(9):2852-2860._x000D_ 2: Ahrenholtz I, Lorenz MG, Wackernagel W. The extracellular nuclease of Serratia marcescens: studies on the activity in vitro and effect on transforming DNA in a groundwater aquifer microcosm Arch Microbiol. 1994;161(2):176-83._x000D_ 3: Franke I, Meiss G, Blecher D, Gimadutdinow O, Urbanke C, Pingoud A. Genetic engineering, production and characterisation of monomeric variants of the dimeric Serratia marcescens endonuclease FEBS Lett. 1998 Apr 3;425(3):517-22._x000D_ 4: Miller MD, Benedik MJ, Sullivan MC, Shipley NS, Krause KL. Crystallization and preliminary crystallographic analysis of a novel nuclease from Serratia marcescens J Mol Biol. 1991 Nov 5;222(1):27-30._x000D_ 5: Meiss G, Gast FU, Pingoud AM. The DNA/RNA non-specific Serratia nuclease prefers double-stranded A-form nucleic acids as substrates J Mol Biol. 1999 May 7;288(3):377-90._x000D_ 6: Miller MD, Krause KL. Identification of the Serratia endonuclease dimer: structural basis and implications for catalysis Protein Sci. 1996 Jan;5(1):24-33._x000D_ 7: Miller MD, Tanner J, Alpaugh M, Benedik MJ, Krause KL. 2.1 A structure of Serratia endonuclease suggests a mechanism for binding to double-stranded DNA Nat Struct Biol. 1994 Jul;1(7):461-8._x000D_ 8: Pommer AJ, Cal S, Keeble AH, Walker D, Evans SJ, Kühlmann UC, Cooper A, Connolly BA, Hemmings AM, Moore GR, James R, Kleanthous C. Mechanism and cleavage specificity of the H-N-H endonuclease colicin E9 J Mol Biol. 2001 Dec 7;314(4):735-49._x000D_ 9: Friedhoff P, Meiss G, Kolmes B, Pieper U, Gimadutdinow O, Urbanke C, Pingoud A. Kinetic analysis of the cleavage of natural and synthetic substrates by the Serratia nuclease Eur J Biochem. 1996 Oct 15;241(2):572-80._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/10834956">10: Lu JJ, Perng CL, Lee SY, Wan CC. Use of PCR with universal primers and restriction endonuclease digestions for detection and identification of common bacterial pathogens in cerebrospinal fluid J Clin Microbiol. 2000 Jun;38(6):2076-80. </a>_x000D_ _x000D_ <strong>Products Related to RNA and DNA Endonuclease from Serratia marcescens can be found at</strong> <a href="https://moleculardepot.com/product-category/Enzymes/"> Enzymes</a>