Chicken anti Green Fluorescent Protein (GFP) [Neisseria gonorrhoeae]
Selected Variant
AGFP
Size: 10 mg
Product Specifications
CAS Number
9007-83-4
UniProt
P42212
Host
Chicken
Conjugation
Egg yolk-derived Purified IgY
Type
Primary Antibodies
Source
Chickens were immunized with recombinant full-length bacterial gonococci Green Fluorescent Protein (GFP) [Neisseria gonorrhoeae] expressed in E. coli cells. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified.
Applications
WB, ELISA
Purification Method
Egg yolk-derived purified IgY (non affinity purified)
Assay Principle
In a Western blot, the anti-GFP used at between 1:3000 and 1:5000 dilution could detect 10 ng of GFP in an A549 cell lysate (see image). Optimal conjµgate working dilutions should be determined for your particular assay conditions.
Concentration
See vial for Concentration
Form
Phosphate buffered saline‚ pH 7,2 with 0,075% sodium azide
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
Shipping Conditions
Ambient Temperature
Storage Conditions
Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
Price
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