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Anti-AIF (NT) Antibody

Rabbit Polyclonal Antibody specific to AIF (NT)

Product Specifications

Product Name Alternative

CNK

Gene Name

CNKSR1

Gene ID

9131

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=CNKSR1">CNKSR1</a>

UniProt

O95381

Accession Number

NP_001124318.2

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 109- 122 at the N-terminus of human AIF.

Target Antigen

Connector enhancer of kinase suppressor of ras 1

Target

AIF (NT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at 1-10ug/mL. <br><br>Immunocytochemistry: use at 2-5ug/mL.Immunocytochemical staining of AIF in Jurkat cells with AIF(NT) antibody at 2 ug/mL. <br><br>These are recommended concentrations. <br><br>Enduser should determine optimal concentrations for their applications. <br><br>Positive control: K562 of Jurkat cells or whole cell lysates.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Product Datasheet

https://www.qedbio.com/wp-content/uploads/pdf/2239.pdf

Specificity

This antibody recognizes human AIF (67 kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

A novel protein that causes chromatin condensation and DNA fragmentation has been designated Apoptosis Inducing Factor (AIF). AIF localizes in mitochondria and translocates to the nucleus when apoptosis is induced. This event is followed by the release of chytochrome c and caspase-9 from mitochondria. AIF is highly conserved between human and mouse and widely expressed in different cell types and tissues.

CAS Number

9007-83-4

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