3mC Recombinant Rabbit Monoclonal Antibody [PSH03-22]
Methylation of DNA can occur non-enzymatically at the nitrogen-three of the cytosine base through spontaneous exposure to endogenous S-adenosyl methionine (SAM). The resulting 3-methylcytosine (3-mC) is mutagenic and must be repaired, which occurs in humans through the base excision repair (BER) or dealkylation via human homologues of the E. coli AlkB protein. 3-methylcytosine is present in human cell lines and increased levels of 3-mC impair proliferation.
Product Specifications
CAS Number
9000-83-3
Host
Rabbit
Species Reactivity
Universal
Immunogen
3-mc-OVA
Isotype
IgG
Clone
PSH03-22
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
ELISA, IF-Cell, Dot Blot
Positive Control
U-2 OS cells treated with 4mM Methyl Methanesulfonate for 1 hour.
Concentration
1 mg/mL
Dilution
IF-Cell: 1:100 ;Dot Blot: 1:2,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
CD106 / VCAM1 (1.4C3), CF405S conjugate, 0.1mg/mL
CD171 / L1CAM (L1 Cell Adhesion Molecule) (UJ127), CF640R conjugate, 0.1mg/mL
HLA-Aw32 & HLA-A25 (MHC I) (CATA-1), CF640R conjugate, 0.1mg/mL
