Endothelin 1 (HRP)

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Crossreactivities determined by competitive RIA: Concentrations (pg/tube) to give 50% B/Bo: Endothelin-1 (human) 1x10e1, Endothelin-2 (human) 4x10e0, Endothelin-3 (human) 1x10e3, Big Endothelin (human) 4x10e0, Endothelin C-terminal hexapeptide <10e5, Atrial natriuretic peptide (human) <10e5, Sarafotoxin S6c 2x10e4