Involucrin (Inv, Invo, IVL) (HRP)

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Recognizes human Involucrin. Epitope: codon 421-568 of human Involucrin at ~66-170kD. Species Crossreactivity: Gorilla, Owl monkey, Porcine, and Canine. Does not react with mouse. In Western Blot of cultured human keratinocytes, reacts with a 120kD protein. It stains the involucrin in a variety of sizes: 170kD in MCF-7 cells, a doublet of ~115kD and 150kD in gorilla and owl monkey, 66kD in canine and a doublet of 105kD in porcine.