Orotic acid-13C,15N2 (monohydrate)

Orotic acid-13C,15N2 (monohydrate) is the 13C and 15N labeled Orotic acid[1]. Orotic acid (6-Carboxyuracil), a precursor in biosynthesis of pyrimidine nucleotides and RNA, is released from the mitochondrial dihydroorotate dehydrogenase (DHODH) for conversion to UMP by the cytoplasmic UMP synthase enzyme. Orotic acid is a marker for measurement in routine newborn screening for urea cycle disorders. Orotic acid can induce hepatic steatosis and hepatomegaly in rats[2][3][4].

Product Specifications

CAS Number

[1346602-15-0]

Product Name Alternative

6-Carboxyuracil-13C,15N2 (monohydrate) ; Vitamin B13-13C,15N2 (monohydrate)

UNSPSC

12352005

Target

Endogenous Metabolite; Nucleoside Antimetabolite/Analog

Type

Isotope-Labeled Compounds

Related Pathways

Cell Cycle/DNA Damage; Metabolic Enzyme/Protease

Applications

Metabolism-protein/nucleotide metabolism

Field of Research

Metabolic Disease

Assay Protocol

https://www.medchemexpress.com/orotic-acid-13c-15n2-monohydrate.html

Purity

99.93

Solubility

DMSO : 55 mg/mL (ultrasonic; warming)

Smiles

OC(C1=CC([15NH][13C]([15NH]1)=O)=O)=O.O

Molecular Formula

C4 13CH6 15N2O5

Molecular Weight

177.09

References & Citations

[1]Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019 Feb;53 (2) :211-216.|[2]Löffler M, et, al. Orotate (orotic acid) : An essential and versatile molecule. Nucleosides Nucleotides Nucleic Acids.|[3]Staretz-Chacham O, et, al. The role of orotic acid measurement in routine newborn screening for urea cycle disorders. J Inherit Metab Dis. 2020 Nov 15.|[4]Durschlag RP, et, al. Orotic acid-induced metabolic changes in the rat. J Nutr. 1980 Apr110 (4) :816-21.