Anti-Phosphotyrosine Antibody

Immunoblotting: use at 1ug/mL. <br><br>Immunohistochemistry: use at 1-10ug/mL. <br><br>Immunofluorescence: use at 1-10ug/mL. <br><br>Positive control: Rat tissue lysate <br><br>These are recommended concentrations; <br><br>Enduser should determine optimal concentrations for their applications.See specific product references below for protocols and more information.

This antibody reacts with phosphotyrosine and detects phosphotyrosine in proteins of unstimulated and stimulated cell lysates. It does not cross-react with phosphoserine or phosphothreonine.

Protein phosphorylation is an important posttranslational modification that serves many key functions in regulation of protein activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by specific protein kinases that remove a phosphate group from ATP and attach it covalently to a recipient protein. Some kinases act on serine and threonine, others act on tyrosine, and many act on all three. Phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity. Antibodies to phosphotyrosine are useful in identifying protein substrates of tyrosine kinase.