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SingleFlowEx CD34 Pacific Blue™

SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

Product Specifications

Certification

RUO (GMP)

Clone

581

Applications

FC (QC tested)

Concentration

0.030 mg/mL

Format

Pacific Blue

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Components

1 vial of monoclonal antibody reagent.The content (1 mL) is sufficient for min. 100 Tests.

References & Citations

*Sanz E, Muñ oz-A N, Monserrat J, Van-Den-Rym A, Escoll P, Ranz I, Alvarez-Mon M, de-la-Hera A: Ordering human CD34+CD10-CD19+ pre/pro-B-cell and CD19- common lymphoid progenitor stages in two pro-B-cell development pathways. Proc Natl Acad Sci U S A. 2010 Mar 30;107 (13) :5925-30., URL: http://www.ncbi.nlm.nih.gov/pubmed/20231472 , *Goardon N, Nikolousis E, Sternberg A, Chu WK, Craddock C, Richardson P, Benson R, Drayson M, Standen G, Vyas P, Freeman S: Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes. Haematologica. 2009 Aug;94 (8) :1160-3., URL: http://www.ncbi.nlm.nih.gov/pubmed/19644143 , *Iwasaki H, Kawamoto A, Ishikawa M, Oyamada A, Nakamori S, Nishimura H, Sadamoto K, Horii M, Matsumoto T, Murasawa S, Shibata T, Suehiro S, Asahara T: Dose-dependent contribution of CD34-positive cell transplantation to concurrent vasculogenesis and cardiomyogenesis for functional regenerative recovery after myocardial infarction. Circulation. 2006 Mar 14;113 (10) :1311-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/16534028, *Ninos JM, Jefferies LC, Cogle CR, Kerr WG: The thrombopoietin receptor, c-Mpl, is a selective surface marker for human hematopoietic stem cells. J Transl Med. 2006 Feb 16;4:9., URL: https://pubmed.ncbi.nlm.nih.gov/16480521/, *Ono F, Sharma BK, Smith CC, Burnett JW, Aurelian L: CD34+ cells in the peripheral blood transport herpes simplex virus DNA fragments to the skin of patients with erythema multiforme (HAEM) .J Invest Dermatol. 2005 Jun;124 (6) :1215-24., URL: https://pubmed.ncbi.nlm.nih.gov/15955097/, *Suárez L, Vidriales MB, García-Laraña J, Sanz G, Moreno MJ, López A, Barrena S, Martínez R, Tormo M, Palomera L, Lavilla E, López-Berges MC, de Santiago M, de Equiza ME, Miguel JF, Orfao A: CD34+ cells from acute myeloid leukemia, myelodysplastic syndromes, and normal bone marrow display different apoptosis and drug resistance-associated phenotypes. Clin Cancer Res. 2004 Nov 15;10 (22) :7599-606., URL: https://www.ncbi.nlm.nih.gov/pubmed/15569991, *Kato S, Ando K, Nakamura Y, Muguruma Y, Sato T, Yabe H, Yabe M, Hattori K, Yasuda Y, Hotta T: Absence of a CD34- hematopoietic precursor population in recipients of CD34+ stem cell transplantation. Bone Marrow Transplant. 2001 Sep;28 (6) :587-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/11607772, *Felschow DM, McVeigh ML, Hoehn GT, Civin CI, Fackler MJ: The adapter protein CrkL associates with CD34. Blood. 2001 Jun 15;97 (12) :3768-75., URL: https://www.ncbi.nlm.nih.gov/pubmed/11389015, *Janowska-Wieczorek A, Marquez LA, Nabholtz JM, Cabuhat ML, Montaño J, Chang H, Rozmus J, Russell JA, Edwards DR, Turner AR: Growth factors and cytokines upregulate gelatinase expression in bone marrow CD34 (+) cells and their transmigration through reconstituted basement membrane. Blood. 1999 May 15;93 (10) :3379-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/10233890, *Ando K, Nakamura Y, Chargui J, Matsuzawa H, Tsuji T, Kato S, Hotta T: Extensive generation of human cord blood CD34 (+) stem cells from Lin (-) CD34 (-) cells in a long-term in vitro system. Exp Hematol. 2000 Jun;28 (6) :690-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10880755, *Bonelli P, Nicolussi P, Manetti R, Antuofermo E, Dattena M: Monoclonal antigen do not cross-react with ovine umbilical cord blood cells. Ital J Anim Sci 2010, vol.9:e6., URL: https://www.tandfonline.com/doi/full/10.4081/ijas.2010.e6, *Use of this product has not been published., URL:

Other References

*Krauter J, Hartl M, Hambach L, Kohlenberg A, Gunsilius E, Ganser A, Heil G: Receptor-mediated endocytosis of CD34 on hematopoietic cells after stimulation with the monoclonal antibody anti-HPCA-1. J Hematother Stem Cell Res. 2001 Dec;10 (6) :863-71., URL: http://www.ncbi.nlm.nih.gov/pubmed/11798512?ordina, *Dao MA, Arevalo J, Nolta JA: Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution. Blood. 2003 Jan 1;101 (1) :112-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/12393633?ordina, *Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Gulati S, Luthra PM: Three-dimensional structure prediction of the interaction of CD34 with the SH3 domain of Crk-L. Stem Cells Dev. 2005 Oct;14 (5) :470-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/16305332?ordina, *Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Luthra PM: Hematopoietic stem cell antigen CD34: role in adhesion or homing. Stem Cells Dev. 2006 Jun;15 (3) :305-13., URL: http://www.ncbi.nlm.nih.gov/pubmed/16846369?ordina

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

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