C-Abl (phospho-Thr735) rabbit pAb

This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t (9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM: 151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [pr

Product Specifications

Background

This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t (9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410) . Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [pr

UniProt

P00519

Swiss Prot

P00519

Reactivity

Human; Rat; Mouse

Immunogen

Synthesized phosho peptide around human c-Abl (Thr735)

Clonality

Polyclonal

Source

Rabbit

Applications

Concentration

1 mg/ml

Dilution

WB 1:1000-2000

Molecular Weight

140(200kd BCR-ABL complex)

Storage Conditions

-20°C/1 year

Observed Molecular Weight

140 (200kd BCR-ABL complex)

Fragment

IgG

Subcellular Location

Cytoplasm, cytoskeleton. Nucleus. Mitochondrion . Shuttles between the nucleus and cytoplasm depending on environmental signals. Sequestered into the cytoplasm through interaction with 14-3-3 proteins. Localizes to mitochondria in response to oxidative stress (By similarity) . .; [Isoform IB]: Nucleus membrane; Lipid-anchor. The myristoylated c-ABL protein is reported to be nuclear.

Other Product Names

Tyrosine-protein kinase ABL1 (EC 2.7.10.2) (Abelson murine leukemia viral oncogene homolog 1) (Abelson tyrosine-protein kinase 1) (Proto-oncogene c-Abl) (p150)