Recombinant Rat PRL Protein (His Tag)
Product Specifications
Background
Prolactin (PRL) is a hormone with multiple actions in the central nervous system (CNS) spanning from physiology to pathology. PRL exerts different actions through its receptors that can be found in both neurons and glial cells (astrocytes, microglia and oligodendrocytes) of the brain. It is generally believed that in vertebrates, prolactin (PRL) is predominantly synthesized and released by pituitary lactotrophs and plays important roles in many physiological processes via activation of PRL receptor (PRLR), including water and electrolyte balance, reproduction, growth and development, metabolism, immuno-modulation, and behavior.
Abbreviation
PRL
Synonyms
Lactotrope; LTH; Luteotropic Hormone; Prolactin precursor; PRL; Prolactin
UniProt
P01237
Accession Number
P01237
Expression System
E.coli
Tag
N-His
Sequence
Leu30-Cys266
Field of Research
Metabolism; Developmental Biology; Neuroscience
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method
Purity
> 90% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
26 kDa
Observed Molecular Weight
28 kDa
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Species
Rat
Available Sizes
Curated Selection
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