Fluorescein-5-maleimide

Fluorescein-5-maleimide (N- (5-Fluoresceinyl) maleimide) is a fluorescent dye. Fluorescein-5-maleimide can be used to detect the redox state of thiols in eukaryotic cells. Fluorescein-5-maleimide can label peptides and is used to detect negatively charged nanoparticles. Fluorescein-5-maleimide can also label actin to explore its interaction with cardiac myosin-binding protein C (cMyBP-C), which helps in developing small molecule modulators for heart failure. Fluorescein-5-maleimide can screen mutant proteins that contain cysteine residues. The excitation wavelength of Fluorescein-5-maleimide is 494 nm, and the emission wavelength is 519 nm[1][2][3][4].

Product Specifications

CAS Number

[75350-46-8]

Product Name Alternative

N- (5-Fluoresceinyl) maleimide

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/Fluorescein-5-maleimide.html

Purity

99.48

Solubility

DMSO : 50 mg/mL (ultrasonic)

Smiles

O=C(C=C1)N(C2=CC3=C(C4(C5=C(OC6=C4C=CC(O)=C6)C=C(O)C=C5)OC3=O)C=C2)C1=O

Molecular Formula

C24H13NO7

Molecular Weight

427.36

Precautions

H302, H315, H319, H335

References & Citations

[1]Wadley AJ, et al. Detecting intracellular thiol redox state in leukaemia and heterogeneous immune cell populations: An optimised protocol for digital flow cytometers. MethodsX. 2018 Nov 1;5:1473-1483. |[2]Akinloye O, et al. Peptide-based fluorescence biosensors for detection/measurement of nanoparticles. Anal Bioanal Chem. 2017 Feb;409 (4) :903-915. |[3]Bunch TA, et al. Drug discovery for heart failure targeting myosin-binding protein C. J Biol Chem. 2023 Dec;299 (12) :105369. |[4]McLachlin DT, et al. A method of screening for mutant proteins containing cysteine residues using fluorescein-5-maleimide. Protein Expr Purif. 1996 May;7 (3) :275-80.