Mouse B7-H3 (2Ig) / CD276 Protein, Fc Tag (MALS verified)

B7 homolog 3 (B7-H3), a member of the immunoglobulin superfamily, is also known CD276, which contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domains. B7-H3 may participate in the regulation of T-cell-mediated immune response. B7-H3 also plays a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. Furthermore, B7-H3 is involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. It could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy.

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 50.1 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

NP_598744

Expression Region

Val 29 - Phe 244

Host

HEK293

Target

B7-H3 (2Ig)

Conjugation

Unconjugated

Tag

C-Fc

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Mouse B7-H3 (2Ig) Protein, Fc Tag (Cat. No. B73-M5255) at 1 μg/mL (100 μL/well) can bind Anti-B7-H3 Antibody, Mouse IgG1 with a linear range of 4-63 ng/mL (QC tested).