Recombinant T4 DNA Ligase

<strong>Recombinant T4 DNA Ligase</strong>_x000D_ <strong>Catalog number:</strong> B2012958_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 20 KU_x000D_ <strong>Molecular Weight or Concentration:</strong> N/A_x000D_ <strong>Supplied as:</strong> Lyophilized Powder_x000D_ <strong>Applications:</strong> molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> -20°C_x000D_ <strong>Keywords:</strong> DNA ligase 4, EC 6.5.1.1, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4._x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Tomkinson AE, Naila T, Khattri Bhandari S. Altered DNA ligase activity in human disease Mutagenesis. 2020 Feb 13;35(1):51-60._x000D_ 2: Gibson DG. Enzymatic assembly of overlapping DNA fragments Methods Enzymol. 2011;498:349-61._x000D_ 3: Lieber MR. The mechanism of double-strand DNA break repair by the nonhomologous DNA end-joining pathway Annu Rev Biochem. 2010;79:181-211._x000D_ 4: Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kühn R. Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells Nat Biotechnol. 2015 May;33(5):543-8._x000D_ 5: Goff NJ, Brenière M, Buehl CJ, de Melo AJ, Huskova H, Ochi T, Blundell TL, Mao W, Yu K, Modesti M, Meek K. Catalytically inactive DNA ligase IV promotes DNA repair in living cells Nucleic Acids Res. 2022 Oct 28;50(19):11058-11071._x000D_ 6: Williamson A, Pedersen H. Recombinant expression and purification of an ATP-dependent DNA ligase from Aliivibrio salmonicida Protein Expr Purif. 2014 May;97:29-36._x000D_ 7: Kimoto M, Soh SHG, Tan HP, Okamoto I, Hirao I. Cognate base-pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase Biopolymers. 2021 Jan;112(1):e23407._x000D_ 8: Francis DB, Kozlov M, Chavez J, Chu J, Malu S, Hanna M, Cortes P. DNA Ligase IV regulates XRCC4 nuclear localization DNA Repair (Amst). 2014 Sep;21:36-42._x000D_ 9: Takahashi F, Funabashi H, Mie M, Endo Y, Sawasaki T, Aizawa M, Kobatake E. Activity-based in vitro selection of T4 DNA ligase Biochem Biophys Res Commun. 2005 Oct 28;336(3):987-93._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/24726358">10: Han L, Masani S, Hsieh CL, Yu K. DNA ligase I is not essential for mammalian cell viability Cell Rep. 2014 Apr 24;7(2):316-320. </a>_x000D_ _x000D_ <strong>Products Related to Recombinant T4 DNA Ligase can be found at</strong> <a href="https://moleculardepot.com/product-category/Enzymes/"> Enzymes</a>