CleanRNA™ Firefly Luciferase-mRNA (low dsRNA)

Firefly luciferase facilitates the conversion of luciferin to oxyluciferin in conditions where ATP, magnesium ions, and oxygen are present, resulting in the emission of bioluminescence at a wavelength of about 560 nm, detectable with a luminometer. CleanRNA™ Firefly Luciferase-mRNA (low dsRNA) is an mRNA is an mRNA that has been modified with a 5' cap1, a 3' poly(A) tail, and N1-Methyl-Pseudouridine Triphosphate (N1-Me-Pseudo UTP). The product is created through an in vitro transcription process using CleaScrip™ T7 RNA polymerase, which generates ultralow dsRNA content in the mRNA solution, thereby improving the in vivo performance of the Firefly Luciferase-mRNA.