Histone H3 Recombinant Mouse Monoclonal Antibody [6-A7-R] - Loading control
The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
HIST1H3A
Swiss Prot
P68431 human, P84243 human, Q16695 human, Q6NXT2 human, Q71DI3 human, P68433 mouse, P84228 mouse, Q6LED0 rat
Cellular Locus
Nucleus.
Host
Mouse
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within Human Histone H3 aa 1-50 / 136.
Isotype
IgG1
Clone
6-A7-R
Conjugation
Non-conjugated
Type
Recombinant Mouse monoclonal Antibody
Applications
WB, IHC-P, IF-Tissue, ChIP
Positive Control
Concentration
1 mg/mL
Dilution
WB: 1:5,000 ;IHC-P: 1:1,000 ;IF-Tissue: 1:200 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 15 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
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