Anti-Ski Antibody

Mouse Monoclonal Antibody specific to SKI

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Proto-oncogene c-Ski

Gene Name

SKI

NCBI Gene ID

UniProt

P12755

Cellular Locus

Nucleus.

Host

Mouse

Reactivity

Human, Mouse, Rat, Chicken, Xenopus

Immunogen

Recombinant v-ski

Target Antigen

Ski oncogene

Target

Ski

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IF, IP

Field of Research

Cancer research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling. {PubMed:19049980}.

Additionnal Information

Immunoblotting: use at 1-5 ug/ml. Predicted molecular weight of v-ski is 55 kDa; full-length ski ranges from ~95 kDa up to ~115 kDa for hyperphosphorylated ski Immunocytochemistry: use at 1-10 ug/ml. ski is a nuclear protein. Immunoprecipitation: use at 1-5 ug/ml. Positive controls: Recombinant cell lines expressing ski proteins.

Storage Conditions

This antibody is stable for at least one (1)year at -70°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes ski protein of human, mouse, rat, chicken, and Xenopus.

Formulation

PBS, pH 7.4

Buffer pH

pH 7.4

Target Background

The ski oncogene family (ski and sno) encodes transcriptional regulators that can affect oncogenic transformation and cellular differenti- ation. ski and sno function as co- activators or co-repressors, modulating transcription by inter- acting with transcription factor complexes. ski interacts with members of the Nuclear Factor I (NFI) family, the retinoic acid receptor, and the nuclear hormone co-regulators NCoR and NCoA62/skip.