Pooled mouse ascites without immunoglobulins
Product Specifications
Background
Ascites obtained from mice bearing different hybridomas, pooled and deleted of all immunoglobulins. It is used as a negative control for ELISA and for indirect immunofluorescence and immunoperoxidase methods, replacing the specific ascites in the test arrangement. The control ascites is used in a dilution comparable to that of the specific ascites.
Assay Principle
The lyophilized ascites is shipped at ambient temperature. After arrival store at +4°C. Prolonged storage may be at -20°C. After opening the vial, the lyophilized content is reconstituted by adding 1 mL of sterile distilled water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted ascites may be stored frozen.
Form
Delipidated, heat inactivated, lyophilized, stable pooled ascites; No preservative added
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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