NCC Antibody (APC)

Rabbit polyclonal to NCC (APC) . NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis. Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1 (1) . In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC. Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension. Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat..

Product Specifications

Product Name Alternative

NCC, SLC12A3, SCYL1, CKb10, MCP-4, MGC17134, NCC-1, NCC1, SCYA13, CK-beta-10, Monocyte chemoattractant protein 4, Monocyte chemotactic protein 4, New CC chemokine 1, Small inducible cytokine A13, Small inducible cytokine subfamily A (Cys-Cys) member 13, Chemokine (C-C)

UniProt

P55017, P55018

Reactivity

Canine, Human, Mouse, Rat

Immunogen

Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal

Target

NCC

Clonality

Polyclonal

Conjugation

APC

Field of Research

Cancer Biology, Neuroscience, Pharmacology & Drug Discovery

Purification

Affinity Purified

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:200)

Molecular Weight

160kDa

Storage Conditions

Conjugated antibodies should be stored according to the product label

Notes

For research use only.

Applications Notes

1 μg/ml was sufficient for detection of NCC3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.