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NKCC2 Antibody (PerCP)

Rabbit polyclonal to NKCC2 (PerCP) . NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell. Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide..

Product Specifications

Product Name Alternative

SLC12A1, sodium potassium chloride cotransporter 2, sodium-potassium-chloride cotransporter isoform 2, BSC1, MBSC1, Na-K-2Cl cotransporter, NKCC2A variant A, RBSC

UniProt

Q5PQV1

Reactivity

Human, Mouse, Rat

Immunogen

Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody designation L224) .

Target

NKCC2

Clonality

Polyclonal

Conjugation

PerCP

Field of Research

Neuroscience

Purification

Protein A Purified

Concentration

1 mg/ml

Dilution

WB (1:1000) ; IHC (1:25)

Molecular Weight

160kDa

Storage Conditions

Conjugated antibodies should be stored according to the product label

Notes

For research use only.

Applications Notes

1 μg/ml was sufficient for detection of NKCC2 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Tested Applications

IHC, WB

NCBI Accession Number

NP_001257546.1

Host or Source

Rabbit

Preservative

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Entrez

25065

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