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NHE3 Antibody (HRP)

Rabbit polyclonal to NHE3 (HRP) . Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine. NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro..

Product Specifications

Product Name Alternative

Sodium / Hydrogen Exchanger 3, SLC9A3, Na+/H+ exchanger 3, isoform 3, Solute carrier family 9 member 3, NHE3MGC126720, MGC126718, solute carrier family 9 (sodium/hydrogen exchanger) member 3, NHE-3, Na (+) /H (+) exchanger 3, Sodium/hydrogen exchanger 3

UniProt

P26433

Reactivity

Mouse, Rat

Immunogen

Synthetic peptide mapping to AA 809 to 831 of rat sequence

Target

NHE3

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Cancer Biology, Neuroscience, Pharmacology & Drug Discovery, Signal Transduction

Purification

Protein A Purified

Concentration

1 mg/ml

Dilution

WB (1:1000) ; IHC (1:25)

Molecular Weight

84kDa

Storage Conditions

Conjugated antibodies should be stored according to the product label

Notes

For research use only.

Applications Notes

1 μg/ml was sufficient for detection of HNE3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Tested Applications

ICC, IF, IHC, WB

NCBI Accession Number

NP_036786

Host or Source

Rabbit

Preservative

73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate

Entrez

24784

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