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GFAP Antibody (PerCP)

Mouse monoclonal to GFAP R416WT (PerCP) . The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia..

Product Specifications

Product Name Alternative

GFAP, Glial fibrillary acidic protein, Intermediate filament protein, Astrocyte, GFAPL, DKFZp459C0729, MGC139638, FLJ45472, AI836096

UniProt

P14136

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP; 100% identical in rat and mouse. > 50% identity with other proteins (Vimentin, Desmin and Peripherin) .

Target

GFAP

Clonality

Monoclonal

Clone

N206B/9 (Formerly sold as S206B-9)

Conjugation

PerCP

Field of Research

Neuroscience

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:1000)

Molecular Weight

50kDa

Storage Conditions

Conjugated antibodies should be stored according to the product label

Notes

For research use only.

Applications Notes

1 μg/ml was sufficient for detection of GFAP R416WT in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Tested Applications

ICC, IF, IHC, WB

NCBI Accession Number

NP_001124491.1

Host or Source

Mouse

Preservative

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Isotype

IgG1

Entrez

2670

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