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VGLUT2 Antibody (Biotin)

Mouse monoclonal to VGlut2 (Biotin) . The ATP-dependent, chloride-sensitive vesicular glutamate transporters (VGLUT) include BNPI (VGLUT1), VGLUT2 (DNPI) and VGLUT3. The brain expresses BNPI (brain specific Na+-dependent inorganic phosphate (Pi) cotransporter) and VGLUT2 in a complementary fashion. The telencephalic regions express BNPI, whereas the lower brainstem and diencephalic regions express VLGUT2. Rat pinealocytes express both BNPI and VGLUT2. The striatum, hippocampus, cerebral cortex and raphe nuclei express VGLUT3 in a small number of neurons. Pancreatic alpha and beta cells express BNPI and VGLUT2 in response to glucose concentrations. Human VGLUT3 shares a 72% sequence homology with VLGUT2 and BNPI..

Product Specifications

Product Name Alternative

VGLUT2, VGluT2, VGLU2, SLC17A6, Solute Carrier Family 17 Member 6, Solute Carrier Family 17 (Sodium-Dependent Inorganic Phosphate Cotransporter) Member 6, Solute Carrier Family 17 (Vesicular Glutamate Transporter) Member 6, Vesicular Glutamate Transporter 2, DNPI, Differentiation-Associated Na (+) -Dependent Inorganic Phosphate Cotransporter, Differentiation-Associated Na-Dependent Inorganic Phosphate Cotransporter, Differentiation-Associated BNPI, Sodium-Dependent Inorganic Phosphate Cotransporter

UniProt

Q9JI12

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2

Target

VGLUT2

Clonality

Monoclonal

Clone

N29/29 (Formerly sold as S29-29)

Conjugation

Biotin

Field of Research

Neuroscience

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100)

Molecular Weight

60kDa

Storage Conditions

Conjugated antibodies should be stored according to the product label

Notes

For research use only.

Applications Notes

1 μg/ml was sufficient for detection of VGLut2 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Tested Applications

ICC, IF, IHC, WB

NCBI Accession Number

NP_445879.1

Host or Source

Mouse

Preservative

136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate

Isotype

IgG1

Entrez

84487

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