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Ubiquitin Antibody

Rabbit Recombinant monoclonal antibody to Ubiquitin

Product Specifications

Product Name Alternative

Polyubiquitin B, RPS27A, UBA52, UBB, UBC, ubiquitin B

UniProt

P0CG48, P0CG47

Reactivity

All

Immunogen

Ubiquitin conjugated lysozyme

Target

Ubiquitin

Clonality

Recombinant

Clone

RGL3R

Conjugation

Unconjugated

Field of Research

Neuroscience, Protein Biochemistry, Signal Transduction

Purification

Protein A Purified

Concentration

1 mg/ml

Dilution

WB (1:1000) ; ELISA (1:1000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

A 1:1000 dilution was sufficient for detection of Ubiquitin in 2 μg of purified poly-ubiquitin chains (Poly-Ubiquitin: Ub1-Ub7 WT Chains K63-linked, k48-linked) by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Tested Applications

ELISA, WB

Host or Source

Rabbit

Preservative

PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.

Isotype

IgG

Entrez

7314

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