Recombinant Chicken Neurofibromin (NF1), partial
Product Specifications
Product Name Alternative
(Neurofibromatosis-related protein NF-1)
Abbreviation
Recombinant Chicken NF1 protein, partial
Gene Name
NF1
UniProt
P35608
Expression Region
1-270aa
Organism
Gallus gallus (Chicken)
Target Sequence
RARVIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQKFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKDSPMHKALFWVAMAVLELDEVNLYSAGTALLEQNLHTLDSLRVFNDKSPEEVFMEIRRPLEWHCKQMDHFVGLNFNSNFNFALVGPGP
Tag
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Type
In Stock Protein
Source
E.coli
Field of Research
Others
Relevance
Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity.
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
35.8 kDa
References & Citations
"Analysis of the sequence and embryonic expression of chicken neurofibromin mRNA." Schafer G.L., Ciment G., Stocker K.M., Baizer L. Mol. Chem. Neuropathol. 18:267-278 (1993)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Product MSDS
https://www.cusabio.com/msds/12934592/
Protein Length
Partial
Available Sizes
Curated Selection
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