Rac1/CDC42 (Phospho Ser71) rabbit pAb

Product Specifications

Product Name Alternative

Ras-related C3 botulinum toxin substrate 1 (Cell migration-inducing gene 5 protein; Ras-like protein TC25; p21-Rac1)

UniProt

P63000/P15153/P60763/P60953

Swiss Prot

P63000/P15153/P60763/P60953

Reactivity

Human; Mouse; Rat

Immunogen

Synthesized peptide derived from human Rac1/CDC42 (Phospho Ser71)

Target

Rac1/CDC42

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA; IHC

Concentration

1 mg/ml

Dilution

WB 1:500-2000; IHC-p 1:50-300; ELISA 2000-20000

Buffer

-20°C/1 year

Storage Conditions

-20°C/1 year

Fragment

IgG

Subcellular Location

Cell membrane ; Lipid-anchor ; Cytoplasmic side . Melanosome . Cytoplasm . Cell projection, lamellipodium . Cell projection, dendrite . Cell junction, synapse . Nucleus . Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (PubMed:1903399) . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065) . Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Localizes to the lamellipodium in a SH3RF1-dependent manner (By similarity) . In macrophages, cytoplasmic location increases upon CSF1 stimulation (By similarity) . Activation by GTP-binding promotes nuclear localization (PubMed:12551911) . .