Rhodamine B hydrazide

Rhodamine B hydrazide is a fluorescent derivative based on rhodamine B, containing the spirocyclic structure of Rhodamine B (HY-Y0016), which can be used to detect copper ions (Cu2+), mercury ions, peroxynitrite, hydroxyl radicals and nitric oxide (NO) [1][2][3]. Excitation/emission wavelength: Conventional detection: 510/578 nm. Sulfite detection: 554 nm absorption, 574 nm emission (due to the formation of Rhodamine B fluorescent product) .

Product Specifications

CAS Number

[74317-53-6]

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/rhodamine-b-hydrazide.html

Concentration

10mM

Purity

99.87

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

CCN(C1=CC2=C(C3(C4=CC=C(C=C4O2)N(CC)CC)C5=C(C(N3N)=O)C=CC=C5)C=C1)CC

Molecular Formula

C28H32N4O2

Molecular Weight

456.58

Precautions

H302, H315, H319, H335

References & Citations

[1]Yang X F, et al. Novel spectrofluorimetric method for the determination of sulfite with rhodamine B hydrazide in a micellar medium[J]. Analytica Chimica Acta, 2002, 456 (1) : 121-128. |[2]Suresh Kumar Kempahanumakkagaari, et al. A new rhodamine B based fluorometric chemodosimeter for Cu2+ ion in aqueous and cellular media. Journal of Luminescence Volume 146, February 2014, Pages 11-17.|[3]Wu CM, et al. Sensitivity evaluation of rhodamine B hydrazide towards nitric oxide and its application for macrophage cells imaging. Anal Chim Acta. 2011 Dec 5;708 (1-2) :141-8.