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Ubiquitin Antibody

Mouse monoclonal antibody to Ubiquitin

Product Specifications

Product Name Alternative

Polyubiquitin B, RPS27A, UBA52, UBB, UBC, ubiquitin B

UniProt

P0CG47

Reactivity

All

Immunogen

Ubiquitin conjugated lysozyme

Target

Ubiquitin

Clonality

Monoclonal

Clone

FK1

Conjugation

Unconjugated

Field of Research

Neuroscience, Protein Biochemistry, Signal Transduction

Purification

Gel filtration

Concentration

1 mg/ml

Dilution

WB (1:10000), ICC/IF (1:100)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

A 1:5000 dilution was sufficient for detection of ubiquitin conjugates in 2.5 μg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Tested Applications

ELISA, ICC, IF, IHC, WB

NCBI Accession Number

BAC56955.1

Host or Source

Mouse

Preservative

PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.

Isotype

IgM

Entrez

7314

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