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PDI Antibody (Biotin)

Mouse monoclonal to EndoPDI (Biotin) . Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia..

Product Specifications

Product Name Alternative

Thioredoxin domain containing 5, ERP46, UNQ364, MGC3178, FLJ21353, FLJ90810, thioredoxin related protein, endothelial protein disulphide isomeras

UniProt

Q8NBS9

Reactivity

Human

Immunogen

Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein

Target

PDI

Clonality

Monoclonal

Clone

2E7/7

Conjugation

Biotin

Field of Research

Cell Biology, Protein Biochemistry, Signal Transduction

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:200)

Molecular Weight

48kDa

Storage Conditions

Conjugated antibodies should be stored according to the product label

Notes

For research use only.

Applications Notes

1 μg/ml was sufficient for detection of EndoPDI in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.

Tested Applications

ELISA, ICC, IF, IHC, IP, WB

NCBI Accession Number

NP_001139021

Host or Source

Mouse

Preservative

136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate

Isotype

IgG2a

Entrez

81567

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