IL-12, Rhesus Macaque (HEK293, His)

Interleukin-12 subunit alpha (IL-12A; IL-12p35), an immune-suppressive cytokine, encodes a subunit of the cytokine IL-12 that acts on T and natural killer cells, and has a broad array of biological activities. IL-12A heterodimerizes with IL-12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine[1][2]. IL-12 Protein, Rhesus Macaque (HEK293, His) is produced in HEK293 cells with a C-Terminal His-tag . It consists of IL-12A (M1-S219) and IL-12B (M1-S328).

IL12A:RNLSVATPGP EMFPCLHHSQ NLLKAASNTL QKARQILEFY PCTSEEIDHE DITKDKTSTV EACLPLELIK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLRSIYEDLK MYQVEFKTMN AKLLRDPKRQ IFLDQNILGV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS IL12B:IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSGEVLGSG KTLTIQVKEF GDAGQYTCHK GGEALSHSLL LLHKKEDGIW STDVLKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SNPQGVTCGA VTLSAERVRG DNKEYEYSVE CQEDSACPAA EERLPIEVMV DAIHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCIQVQGKSK REKKDRIFTD KTSATVICRK NASFSVQAQD RYYSSSWSEW ASVPCS

[1]Ivy M Dambuza, et al. IL-12p35 induces expansion of IL-10 and IL-35-expressing regulatory B cells and ameliorates autoimmune disease. Nat Commun. 2017 Sep 28;8(1):719.|[2]Jin Kyeong Choi, et al. IL-12p35 Inhibits Neuroinflammation and Ameliorates Autoimmune Encephalomyelitis. Front Immunol. 2017 Oct 5;8:1258.|[3]D De Wit, et al. Helper T-cell responses elicited by Der p 1-pulsed dendritic cells and recombinant IL-12 in atopic and healthy subjects. J Allergy Clin Immunol. 2000 Feb;105(2 Pt 1):346-52.|[4]A A Ansari, et al. Administration of recombinant rhesus interleukin-12 during acute simian immunodeficiency virus (SIV) infection leads to decreased viral loads associated with prolonged survival in SIVmac251-infected rhesus macaques. J Virol. 2002 Feb;76(4):1731-43.