Recombinant Mouse Mitochondrial glutamate carrier 2 (Slc25a18)
Product Specifications
Product Name Alternative
(GC-2) (Glutamate/H (+) symporter 2) (Solute carrier family 25 member 18)
Abbreviation
Recombinant Mouse Slc25a18 protein
Gene Name
Slc25a18
UniProt
Q9DB41
Expression Region
1-320aa
Organism
Mus musculus (Mouse)
Target Sequence
MIACRMSSQDLSISAKLINGGIAGLVGVTCVFPIDLAKTRLQNQQGKDVYRGMTDCLMKTARAEGFLGMYRGAAVNLTLVTPEKAIKLAANDFLRQLLMQDGTQRNLKMEMLAGCGAGICQVVITCPMEMLKIQLQDAGRLAVCHQASASATPTSRPYSTGSTSTHRRPSATLIARELLRTQGLSGLYRGLGATLLRDIPFSIIYFPLFANLNQLGVSELTGKASFTHSFVAGCTAGSVAAVAVTPLDVLKTRIQTLKKGLGEDTYSGVTDCARKLWTQEGPAAFMKGAGCRALVIAPLFGIAQGVYFIGIGERILKCFE-
Tag
N-terminal 10xHis-tagged
Type
CF Transmembrane Protein & Developed Protein
Source
In vitro E.coli expression system
Field of Research
Cancer
Relevance
Responsible for the transport of glutamate from the cytosol into the mitochondrial matrix with the concomitant import of a proton (symport system) .
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
35.7 kDa
References & Citations
"Trans-synaptic interaction of GluRdelta2 and Neurexin through Cbln1 mediates synapse formation in the cerebellum." Uemura T., Lee S.J., Yasumura M., Takeuchi T., Yoshida T., Ra M., Taguchi R., Sakimura K., Mishina M. Cell 141:1068-1079 (2010)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length
Available Sizes
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