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Adenosine Colorimetric Assay Kit

Product Specifications

Type

Colorimetric method

Field of Research

Metabolic Diseases

Assay Principle

Adenosine is an endogenous nucleoside distributed throughout human cells. It can directly enter the myocardium and be phosphorylated to produce adenosine, which is involved in myocardial energy metabolism, and also participates in the dilation of coronary vessels and increase blood flow. Adenosine has physiological effects on the cardiovascular system and many other systems and tissues of the body. It also promotes the growth of human hair follicles and the proliferation of dermal papilla cells in vitro. The detection principle of this kit is that the substances produced by the enzyme-catalyzed adenosine reaction consume NADH, the OD value of the system decreases at 340 nm, and the content of adenosine can be calculated by calculating the decline rate at 340 nm.

Assay Performance Time

40 min

Sample Type

Serum; Plasma; Animal and plant tissue; Cell

Detection Range

0.027-1.000 mmol/L

Sensitivity

0.027 mmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

1.9-6.8%

Intra CV

3.5-5.8%

Detection Instrument

Microplate reader

Frequently Asked Questions

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