Recombinant Schistosoma mansoni Antigenic integral membrane glycoprotein, partial

Product Specifications

Product Name Alternative

Antigen Sm25

Abbreviation

UniProt

P23126

Expression Region

69-160aa

Organism

Schistosoma mansoni (Blood fluke)

Target Sequence

VNSEENSNSIITDEDYDHYNSSLDSSNNVKHSQEAFHRNSDPDGFPEYEFLNETSIEIKEELGQELHQLQLILDELSRRIRATPNSANKYMK

Tag

N-terminal 6xHis-Trx-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Major antigen in the surface tegument.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

28.9 kDa

References & Citations

"Structure of Sm25, an antigenic integral membrane glycoprotein of adult Schistosoma mansoni." Omer Ali P., Jeffs S.A., Meadows H.M., Hollyer T., Owen C., Hackett F., Smithers S.R., Simpson A.J.G. Mol. Biochem. Parasitol. 45:215-222 (1991)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.